近日，腐植酸科学与工程研究团队/天然产物制药课题组（即本团队）在植物学类SCI期刊《Plants》杂志(JCR一区，中科院二区，IF=4.5)在线发表了题为“The Influence of Sodium Humate on the Biosynthesis and Contents of Flavonoid Constituents in Lemons（腐植酸钠对柠檬中类黄酮成分生物合成和含量的影响）”的文章。通讯作者为张敉教授，第一作者为课题组在读硕士生徐念澳同学，其他学生作者还包括已毕业的张汉琪、任有地两位同学。

摘要内容如下：Sodium humate (SH) is the sodium salt of humic acid. Our previous research has demonstrated that SH has the ability to enhance the levels of total flavonoids in various parts of lemons, including the leaves, peels, pulps, and seeds, thereby improving the quality of lemons. In the current study, the regulation effect of SH on the biosynthesis and content of lemon flavonoid compounds was examined using transcriptome sequencing technology and flavonoid metabolomic analysis. Following SH treatment, the transcriptome sequencing analysis revealed 320 differentially expressed genes (DEGs) between samples treated with SH and control (CK) samples, some of which were associated with the phenylalanine pathway by KEGG annotation analysis. The levels of seven flavonoid compounds identified in lemon peels were observed to increase, and eriocitrin and isoorientin were identified as differential metabolites (DMs, VIP > 1) using OPLS-DA analysis. The integrated analysis of transcriptomics and flavonoid metabolomics indicates that SH treatment induces alterations in gene expression and metabolite levels related to flavonoid synthesis. Specifically, SH influences flavonoid biosynthesis by modulating the activity of key enzymes in the phenylalanine pathway, including HCT (O-hydroxycinnamoyltransferase) and F5H (ferulate-5-hydroxylase). （腐植酸钠（SH）是腐植酸的钠盐。我们之前的研究表明，SH能够提高柠檬叶、皮、果肉和种子中总黄酮的水平，从而提高柠檬的品质。本研究利用转录组测序技术和类黄酮代谢组学分析，研究了SH对柠檬类黄酮化合物生物合成和含量的调控作用。在SH处理后，转录组测序分析显示，SH处理的样品与对照（CK）样品之间存在320个差异表达基因（DEGs），其中一些基因通过KEGG注释分析与苯丙氨酸途径相关。经OPLS-DA分析，柠檬皮中7种黄酮类化合物含量均升高，并鉴定为差异代谢产物（DMs, VIP > 1）。转录组学和类黄酮代谢组学的综合分析表明，SH处理诱导了与类黄酮合成相关的基因表达和代谢物水平的改变。具体来说，SH通过调节苯丙氨酸途径中关键酶的活性来影响类黄酮的生物合成，包括HCT（O-羟基肉桂酰基转移酶）和 F5H（阿魏酸-5-羟化酶）。）

文章链接：https://www.mdpi.com/2223-7747/13/20/2888